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786 o cas9 cells  (Genecopoeia)


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    Genecopoeia 786 o cas9 cells
    ( A ) Left: Representative images of crystal violet staining of HEK293FT cells 72 hours after transfection with an empty vector control (EV), WT-ACSS2, or ΔT376K catalytically inactive mutant. Right: Bar graph showing quantification of 3 independent experiments in HEK293FT cells. Statistical significance was determined using a 1-way ANOVA and Tukey’s multiple-comparison test (* P < 0.05; ** P < 0.01). Data are represented as mean ± SD. ( B ) Western blot images showing expression of HIF-2α ( n = 3), ACSS2 ( n = 3), and actin ( n = 3) in 786-O <t>Cas9</t> sgControl and sgACSS2 cells transfected with EV, WT-ACSS2, or ΔT376K. ( C ) Left: Representative images of crystal violet staining of 786-O Cas9 cells stably transduced to express sgControl or sgACSS2 72 hours after transfection with an empty vector control (EV), WT-ACSS2, or ΔT376K catalytically inactive mutant. Right: Bar graph showing quantification of 3 independent experiments in 786-O Cas9 cells. Statistical significance was determined using a 2-way ANOVA and Tukey’s multiple-comparison test (* P < 0.05; ** P < 0.01). Data are represented as mean ± SD. ( D ) Representative images from day 4 of a tumor sphere formation assay where 786-O Cas9 sgControl and sgACSS2 cells were transfected with EV, WT-ACSS2, or ΔT376K.
    786 O Cas9 Cells, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 94/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/786+o+cas9+cells/Human+cell+line+786-O+stably+expressing+Cas9%2C+randomly+inserted%2C+single+clone/pmc11178540-202-0-6
    Average 94 stars, based on 7 article reviews
    786 o cas9 cells - by Bioz Stars, 2026-10
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    Images

    1) Product Images from "HIF-2 α expression and metabolic signaling require ACSS2 in clear cell renal cell carcinoma"

    Article Title: HIF-2 α expression and metabolic signaling require ACSS2 in clear cell renal cell carcinoma

    Journal: The Journal of Clinical Investigation

    doi: 10.1172/JCI164249

    ( A ) Left: Representative images of crystal violet staining of HEK293FT cells 72 hours after transfection with an empty vector control (EV), WT-ACSS2, or ΔT376K catalytically inactive mutant. Right: Bar graph showing quantification of 3 independent experiments in HEK293FT cells. Statistical significance was determined using a 1-way ANOVA and Tukey’s multiple-comparison test (* P < 0.05; ** P < 0.01). Data are represented as mean ± SD. ( B ) Western blot images showing expression of HIF-2α ( n = 3), ACSS2 ( n = 3), and actin ( n = 3) in 786-O Cas9 sgControl and sgACSS2 cells transfected with EV, WT-ACSS2, or ΔT376K. ( C ) Left: Representative images of crystal violet staining of 786-O Cas9 cells stably transduced to express sgControl or sgACSS2 72 hours after transfection with an empty vector control (EV), WT-ACSS2, or ΔT376K catalytically inactive mutant. Right: Bar graph showing quantification of 3 independent experiments in 786-O Cas9 cells. Statistical significance was determined using a 2-way ANOVA and Tukey’s multiple-comparison test (* P < 0.05; ** P < 0.01). Data are represented as mean ± SD. ( D ) Representative images from day 4 of a tumor sphere formation assay where 786-O Cas9 sgControl and sgACSS2 cells were transfected with EV, WT-ACSS2, or ΔT376K.
    Figure Legend Snippet: ( A ) Left: Representative images of crystal violet staining of HEK293FT cells 72 hours after transfection with an empty vector control (EV), WT-ACSS2, or ΔT376K catalytically inactive mutant. Right: Bar graph showing quantification of 3 independent experiments in HEK293FT cells. Statistical significance was determined using a 1-way ANOVA and Tukey’s multiple-comparison test (* P < 0.05; ** P < 0.01). Data are represented as mean ± SD. ( B ) Western blot images showing expression of HIF-2α ( n = 3), ACSS2 ( n = 3), and actin ( n = 3) in 786-O Cas9 sgControl and sgACSS2 cells transfected with EV, WT-ACSS2, or ΔT376K. ( C ) Left: Representative images of crystal violet staining of 786-O Cas9 cells stably transduced to express sgControl or sgACSS2 72 hours after transfection with an empty vector control (EV), WT-ACSS2, or ΔT376K catalytically inactive mutant. Right: Bar graph showing quantification of 3 independent experiments in 786-O Cas9 cells. Statistical significance was determined using a 2-way ANOVA and Tukey’s multiple-comparison test (* P < 0.05; ** P < 0.01). Data are represented as mean ± SD. ( D ) Representative images from day 4 of a tumor sphere formation assay where 786-O Cas9 sgControl and sgACSS2 cells were transfected with EV, WT-ACSS2, or ΔT376K.

    Techniques Used: Staining, Transfection, Plasmid Preparation, Control, Mutagenesis, Comparison, Western Blot, Expressing, Stable Transfection, Tube Formation Assay

    Related Articles

    Staining:

    Article Title: HIF-2 α expression and metabolic signaling require ACSS2 in clear cell renal cell carcinoma
    Article Snippet: A498, 786-O, and 769-P cell lines were obtained from ATCC. .. 786-O Cas9 cells were purchased from Genecopoeia. .. Before experimentation, all cell lines were tested for mycoplasma co

    Article Title: HIF-2α expression and metabolic signaling require ACSS2 in clear cell renal cell carcinoma
    Article Snippet: A498, 786-O, and 769-P cell lines were obtained from ATCC. .. 786-O Cas9 cells were purchased from Genecopoeia. .. Before experimentation, all cell lines were tested for mycoplasma co

    Transfection:

    Article Title: HIF-2 α expression and metabolic signaling require ACSS2 in clear cell renal cell carcinoma
    Article Snippet: A498, 786-O, and 769-P cell lines were obtained from ATCC. .. 786-O Cas9 cells were purchased from Genecopoeia. .. Before experimentation, all cell lines were tested for mycoplasma co

    Article Title: HIF-2α expression and metabolic signaling require ACSS2 in clear cell renal cell carcinoma
    Article Snippet: A498, 786-O, and 769-P cell lines were obtained from ATCC. .. 786-O Cas9 cells were purchased from Genecopoeia. .. Before experimentation, all cell lines were tested for mycoplasma co

    Plasmid Preparation:

    Article Title: HIF-2 α expression and metabolic signaling require ACSS2 in clear cell renal cell carcinoma
    Article Snippet: A498, 786-O, and 769-P cell lines were obtained from ATCC. .. 786-O Cas9 cells were purchased from Genecopoeia. .. Before experimentation, all cell lines were tested for mycoplasma co

    Article Title: HIF-2α expression and metabolic signaling require ACSS2 in clear cell renal cell carcinoma
    Article Snippet: A498, 786-O, and 769-P cell lines were obtained from ATCC. .. 786-O Cas9 cells were purchased from Genecopoeia. .. Before experimentation, all cell lines were tested for mycoplasma co

    Control:

    Article Title: HIF-2 α expression and metabolic signaling require ACSS2 in clear cell renal cell carcinoma
    Article Snippet: A498, 786-O, and 769-P cell lines were obtained from ATCC. .. 786-O Cas9 cells were purchased from Genecopoeia. .. Before experimentation, all cell lines were tested for mycoplasma co

    Article Title: HIF-2α expression and metabolic signaling require ACSS2 in clear cell renal cell carcinoma
    Article Snippet: A498, 786-O, and 769-P cell lines were obtained from ATCC. .. 786-O Cas9 cells were purchased from Genecopoeia. .. Before experimentation, all cell lines were tested for mycoplasma co

    Mutagenesis:

    Article Title: HIF-2 α expression and metabolic signaling require ACSS2 in clear cell renal cell carcinoma
    Article Snippet: A498, 786-O, and 769-P cell lines were obtained from ATCC. .. 786-O Cas9 cells were purchased from Genecopoeia. .. Before experimentation, all cell lines were tested for mycoplasma co

    Article Title: HIF-2α expression and metabolic signaling require ACSS2 in clear cell renal cell carcinoma
    Article Snippet: A498, 786-O, and 769-P cell lines were obtained from ATCC. .. 786-O Cas9 cells were purchased from Genecopoeia. .. Before experimentation, all cell lines were tested for mycoplasma co

    Comparison:

    Article Title: HIF-2 α expression and metabolic signaling require ACSS2 in clear cell renal cell carcinoma
    Article Snippet: A498, 786-O, and 769-P cell lines were obtained from ATCC. .. 786-O Cas9 cells were purchased from Genecopoeia. .. Before experimentation, all cell lines were tested for mycoplasma co

    Article Title: HIF-2α expression and metabolic signaling require ACSS2 in clear cell renal cell carcinoma
    Article Snippet: A498, 786-O, and 769-P cell lines were obtained from ATCC. .. 786-O Cas9 cells were purchased from Genecopoeia. .. Before experimentation, all cell lines were tested for mycoplasma co

    Western Blot:

    Article Title: HIF-2 α expression and metabolic signaling require ACSS2 in clear cell renal cell carcinoma
    Article Snippet: A498, 786-O, and 769-P cell lines were obtained from ATCC. .. 786-O Cas9 cells were purchased from Genecopoeia. .. Before experimentation, all cell lines were tested for mycoplasma co

    Article Title: HIF-2α expression and metabolic signaling require ACSS2 in clear cell renal cell carcinoma
    Article Snippet: A498, 786-O, and 769-P cell lines were obtained from ATCC. .. 786-O Cas9 cells were purchased from Genecopoeia. .. Before experimentation, all cell lines were tested for mycoplasma co

    Expressing:

    Article Title: HIF-2 α expression and metabolic signaling require ACSS2 in clear cell renal cell carcinoma
    Article Snippet: A498, 786-O, and 769-P cell lines were obtained from ATCC. .. 786-O Cas9 cells were purchased from Genecopoeia. .. Before experimentation, all cell lines were tested for mycoplasma co

    Article Title: HIF-2α expression and metabolic signaling require ACSS2 in clear cell renal cell carcinoma
    Article Snippet: A498, 786-O, and 769-P cell lines were obtained from ATCC. .. 786-O Cas9 cells were purchased from Genecopoeia. .. Before experimentation, all cell lines were tested for mycoplasma co

    Stable Transfection:

    Article Title: HIF-2 α expression and metabolic signaling require ACSS2 in clear cell renal cell carcinoma
    Article Snippet: A498, 786-O, and 769-P cell lines were obtained from ATCC. .. 786-O Cas9 cells were purchased from Genecopoeia. .. Before experimentation, all cell lines were tested for mycoplasma co

    Article Title: HIF-2α expression and metabolic signaling require ACSS2 in clear cell renal cell carcinoma
    Article Snippet: A498, 786-O, and 769-P cell lines were obtained from ATCC. .. 786-O Cas9 cells were purchased from Genecopoeia. .. Before experimentation, all cell lines were tested for mycoplasma co

    Tube Formation Assay:

    Article Title: HIF-2 α expression and metabolic signaling require ACSS2 in clear cell renal cell carcinoma
    Article Snippet: A498, 786-O, and 769-P cell lines were obtained from ATCC. .. 786-O Cas9 cells were purchased from Genecopoeia. .. Before experimentation, all cell lines were tested for mycoplasma co

    Article Title: HIF-2α expression and metabolic signaling require ACSS2 in clear cell renal cell carcinoma
    Article Snippet: A498, 786-O, and 769-P cell lines were obtained from ATCC. .. 786-O Cas9 cells were purchased from Genecopoeia. .. Before experimentation, all cell lines were tested for mycoplasma co



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    ( A ) Left: Representative images of crystal violet staining of HEK293FT cells 72 hours after transfection with an empty vector control (EV), WT-ACSS2, or ΔT376K catalytically inactive mutant. Right: Bar graph showing quantification of 3 independent experiments in HEK293FT cells. Statistical significance was determined using a 1-way ANOVA and Tukey’s multiple-comparison test (* P < 0.05; ** P < 0.01). Data are represented as mean ± SD. ( B ) Western blot images showing expression of HIF-2α ( n = 3), ACSS2 ( n = 3), and actin ( n = 3) in 786-O <t>Cas9</t> sgControl and sgACSS2 cells transfected with EV, WT-ACSS2, or ΔT376K. ( C ) Left: Representative images of crystal violet staining of 786-O Cas9 cells stably transduced to express sgControl or sgACSS2 72 hours after transfection with an empty vector control (EV), WT-ACSS2, or ΔT376K catalytically inactive mutant. Right: Bar graph showing quantification of 3 independent experiments in 786-O Cas9 cells. Statistical significance was determined using a 2-way ANOVA and Tukey’s multiple-comparison test (* P < 0.05; ** P < 0.01). Data are represented as mean ± SD. ( D ) Representative images from day 4 of a tumor sphere formation assay where 786-O Cas9 sgControl and sgACSS2 cells were transfected with EV, WT-ACSS2, or ΔT376K.
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    Image Search Results


    ( A ) Left: Representative images of crystal violet staining of HEK293FT cells 72 hours after transfection with an empty vector control (EV), WT-ACSS2, or ΔT376K catalytically inactive mutant. Right: Bar graph showing quantification of 3 independent experiments in HEK293FT cells. Statistical significance was determined using a 1-way ANOVA and Tukey’s multiple-comparison test (* P < 0.05; ** P < 0.01). Data are represented as mean ± SD. ( B ) Western blot images showing expression of HIF-2α ( n = 3), ACSS2 ( n = 3), and actin ( n = 3) in 786-O Cas9 sgControl and sgACSS2 cells transfected with EV, WT-ACSS2, or ΔT376K. ( C ) Left: Representative images of crystal violet staining of 786-O Cas9 cells stably transduced to express sgControl or sgACSS2 72 hours after transfection with an empty vector control (EV), WT-ACSS2, or ΔT376K catalytically inactive mutant. Right: Bar graph showing quantification of 3 independent experiments in 786-O Cas9 cells. Statistical significance was determined using a 2-way ANOVA and Tukey’s multiple-comparison test (* P < 0.05; ** P < 0.01). Data are represented as mean ± SD. ( D ) Representative images from day 4 of a tumor sphere formation assay where 786-O Cas9 sgControl and sgACSS2 cells were transfected with EV, WT-ACSS2, or ΔT376K.

    Journal: The Journal of Clinical Investigation

    Article Title: HIF-2 α expression and metabolic signaling require ACSS2 in clear cell renal cell carcinoma

    doi: 10.1172/JCI164249

    Figure Lengend Snippet: ( A ) Left: Representative images of crystal violet staining of HEK293FT cells 72 hours after transfection with an empty vector control (EV), WT-ACSS2, or ΔT376K catalytically inactive mutant. Right: Bar graph showing quantification of 3 independent experiments in HEK293FT cells. Statistical significance was determined using a 1-way ANOVA and Tukey’s multiple-comparison test (* P < 0.05; ** P < 0.01). Data are represented as mean ± SD. ( B ) Western blot images showing expression of HIF-2α ( n = 3), ACSS2 ( n = 3), and actin ( n = 3) in 786-O Cas9 sgControl and sgACSS2 cells transfected with EV, WT-ACSS2, or ΔT376K. ( C ) Left: Representative images of crystal violet staining of 786-O Cas9 cells stably transduced to express sgControl or sgACSS2 72 hours after transfection with an empty vector control (EV), WT-ACSS2, or ΔT376K catalytically inactive mutant. Right: Bar graph showing quantification of 3 independent experiments in 786-O Cas9 cells. Statistical significance was determined using a 2-way ANOVA and Tukey’s multiple-comparison test (* P < 0.05; ** P < 0.01). Data are represented as mean ± SD. ( D ) Representative images from day 4 of a tumor sphere formation assay where 786-O Cas9 sgControl and sgACSS2 cells were transfected with EV, WT-ACSS2, or ΔT376K.

    Article Snippet: 786-O Cas9 cells were purchased from Genecopoeia.

    Techniques: Staining, Transfection, Plasmid Preparation, Control, Mutagenesis, Comparison, Western Blot, Expressing, Stable Transfection, Tube Formation Assay